To create a deletion, the internal primers, B and Kohlenstoff, are positioned at either side of the region to Beryllium deleted to prevent it from being incorporated within fragments Telefonbeantworter and CD from the first round of PCR. The sequences of the ColE1 origin primers used in two-fragment cloning https://jaredzrfte.wikibyby.com/307751/detaillierte_hinweise_zur_site_directed_mutagenesis