POD reacting with hydrogen peroxide (H2O2) catalyzes oxidative condensation of tyramide to make really reactive intermediates, tyramide radicals. The activated tyramide covalently binds to electron-abundant moieties such as tyrosine, phenylalanine and tryptophan at or near the POD. The limited 50 %-lifetime of the tyramide radicals restricts the deposition of tyramide3. https://christianx110kwi4.wikicommunication.com/user